Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
L-ethionine | 37°C, pH 8.0 | Pseudomonas putida | |
1.7 | - |
L-methionine | 37°C, pH 8.0 | Pseudomonas putida | |
30 | - |
L-homoserine | 37°C, pH 8.0 | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + H2O | Pseudomonas putida | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant METase | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ethionine + H2O | 60% of activity with L-methinine | Pseudomonas putida | ethanethiol + NH3 + 2-oxobutanoate | - |
? | |
L-homoserine | - |
Pseudomonas putida | NH3 + 2-oxobutanoate | - |
? | |
L-methionine + H2O | - |
Pseudomonas putida | methanethiol + NH3 + 2-oxobutanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
METase | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |